Cedric Hardwicke
Dr. Ludwig Frankenstein / Dr. Henry Frankenstein (ghostly image)
Ralph Bellamy
Erik Ernst
Lionel Atwill
Dr. Bohmer
Bela Lugosi
Ygor
Evelyn Ankers
Elsa Frankenstein
Lon Chaney Jr.
The Frankenstein Monster
Barton Yarborough
Dr. Kettering
Doris Lloyd
Martha the Housekeeper
Leyland Hodgson
Chief Constable
Olaf Hytten
Adolph Hussman